L-Glutamic Acid CAS 56-86-0 (H-Glu-OH) Assay 98.5~100.5% Factory High Quality

Transmittance of Solution
Take this product l. After adding 2mol/L hydrochloric acid solution (20ml) to be dissolved, measure the transmittance at the wavelength of 430nm by UV-visible spectrophotometry (General rule 0401), and the transmittance shall not be less than 98.0%.
Chloride
Take 0.30g of this product and check it according to law (General rule 0801). Compared with the control solution made of 6.0ml of standard sodium chloride solution, it should not be more concentrated (0.02%).
Sulfate
Take 0.50g of this product, add 2ml of dilute hydrochloric acid and 5ml of water, shake to dissolve, check according to law (General rule 0802), and compare with the control solution made of standard potassium sulfate solution, no more concentrated (0.02%).
Ammonium Salt
Take 0.10g of this product and check it according to law (General rule 0808). Compared with the control solution made of 0.02% of standard ammonium chloride solution, it shall not be deeper ().
Other Amino Acids
Take this product, add 0.5mol/L hydrochloric acid solution to dissolve and dilute to prepare a solution containing about 10 mg per 1ml as a test solution; Take 1ml for precision measurement, in a 0.5 measuring flask, dilute to the scale with mol/L hydrochloric acid solution, shake well, and use as a control solution, A 0.5mol/L hydrochloric acid solution was added, dissolved and diluted to prepare a solution containing about 10 mg of glutamic acid and MG of aspartic acid per 1ml, respectively, as a system-suitable solution. According to the thin layer chromatography (General 0502) test, draw 5ul of each of the above three solutions, respectively, on the same silica gel G thin layer plate, with n-butanol-water-glacial acetic acid (2:1:1) to develop, spread, dry, spray with ninhydrin in acetone solution (1-50), heat at 80°C until spots appear, and immediately inspect. The control solution should show a clear spot, and the system applicable solution should show two completely separated spots. If the test solution shows impurity spots, the color should not be deeper (0.5%) than the main spot of the control solution.
Loss on Drying
Take this product, dry to constant weight at 105℃, weight loss shall not exceed 0.5% (General rule 0831).
Residue on Ignition (as Sulfated)
Take l.0g of this product and check it according to law (General rule 0841). The residue left shall not exceed 0.1%.
Iron Salt
Take 2.0g of this product, add 6mL of dilute hydrochloric acid and an appropriate amount of water, heat to dissolve, cool, add water to 25ml, and check according to law (General rule 0807), it should not be deeper (0.0005%) than the control solution made of standard iron solution.
Heavy Metals
The residue left under the item of taking the ignition residue shall not contain more than 10 parts per million of heavy metal when examined by law (General Principles 0821, Law II).
Arsenic Salt
Take 2.0g of this product, add 5ml of hydrochloric acid and 23ml of water to dissolve, check according to law (General Principles 0822 first law), should comply with the provisions (0.0001%).
Pyrogen
This product is diluted with sodium chloride injection to make a solution containing 20mg per lml, heated to dissolve, let cool to 37°C, and check according to law (General rule 1142), dose according to the rabbit body weight per lkg injection 10ml, should comply with the provisions. (For injection)
56-86-0 - Content determination
Take this product about 0.25g, precision weighing, add boiling water 50ml to dissolve, let cool, add 5 drops of bromothymol blue indicator solution, and use sodium hydroxide titration solution (0.1 mol/L) titration to the solution from yellow to blue-green. Each 1 ml of sodium hydroxide titration solution (0.1 mol/L) corresponds to 14.71mg of C5H9N04.